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Really it is a great moment for all of us that a student of this prestigious Institution has adorned the office of Chief Justice of the country.

Justice Mishra. No slides found, please add some slides. Ftnallv after the elution stood for 2 mu drop of urea C to the 1 ml.

Froa 0 mL of potasaum iodide solution b then added to the taming 'up to 1 mg. After addmi tie permanganate, rotate the solution gently upon the sides d the flask and heat tho saraples for two romutes Then add 2 dn?

The iodme is liberated from the lodato by tho addition of iodide and titrated with sodrum thiosulfate.

The contents must be clear and colorless, otheiTiiae a few more drops of sulfunc acid must be added The hot flask is put back on the flame and dDuto KNlnOi solution 3 IS added till the pink color remains.

S mg of phosphorus are added to 80 ml of 10 per cent tnchloracetio acid solution m a mh volumetno [lask. The solution keeps for approximately 2 weeks, when kept cold and in the dark icebox pToadurt A test tube with ground glass top is filial with 8 ml of tnchlor acetic acid and 2 mL of oxalaCed blood is added with ahaking After continued shakmg it b filtered through an ash-free filter Five mL of filtrate correspondmg to 1 ml of blood 1 mL of molybdate and 0 4 mL of ammosulfomo add reagent are pipetted into a 10 mL vdu mstno flfiak, water added up to the mark and the contenta weD muted.

At the same tune 6 ml of the working standard phosphate solutioa a or is pipetted into a 10 mL volumetne OMk, 1 mJ of molybdate and 0 4 ml of ammoaulfoDio acid reagent are added, and made up with water to the mark.

P y w colorimeter reading. The rate of dilution can be found by dotermiaing the concentratjon of the stock solution as follows mto a 60 mL measuring flask is placed 0 1 ml of SdCIj and made up to the mark with distilled water Test tubes of 2J5 cm.

With stining 0. Further detenmnatioa and calculatMn is earned out according to directions for the deter mmatlon of phosphorus m serum.

Filter if cloudy For all other reagents see determination of inorganic phosphorus. If the digestlonismcomplete a black residue remainfl In this case 0J2 ml of fresh HtOt must be added and the digestion continued.

Unknown and standard are now dOutod to the 8 ml mark with redistilled water and 1 ml of molybdate reagent 3 and 1 mb of dilute SnDi solution is added.

If tho Hdhge colorimeter is used the method is the same as de- scribed above Solution 0 serves as standard. For all other reagents see determination of total acid-soluble phosphorus Proaditn Into a 10 ml volumetno flask ore placed approximately 0 ml of the alcohol-ether mixture.

Slowly and with shaking 0. Values of 8 mg pier cent and above can be found m this disease Similar changes occur m nephrosclerosis, in cystic kidneys, in pyonephrosis, hjilronephrosis, ond m tuberculosis of the kidney 4 During healing of bone fractures the phosphate levd is in creased.

An mcrcoso m phosphorus is a sign of kidney msuflicicncy and is often found together with a decrease of alkali- reservT Values of 8 mg per coot end above can be found in this disease Similar changes occur in nephrosclerosis, m ej'stk kMnoys, m pjoncphroeifl, hvdroncpbrosis and m tuberculosis of the kidney 4 Durmg healing of bone fractures tho phosphate level is lu creased.

Durmg this penod values may vary between 6 and 7 mg per cent Low scrum levels are found m I Rickets In children values between 1 and 2 mg pcf have been encountered.

ExampU 0. The air should bo sucked through the mixture kept in a narrow tube. Both solutions can be kept on ice for a long time. Therefore it IB difBcult to give exact dirootions for the preparation of a standard solution.

This procedure is repeated tonos until the supernatant is colorless. After removal of the water as described above, 6 ml of ceno reagent 2 IS added and the tubes ore heated m a boiling water bath for approximately tuo minutes to dissolve the precipitate Thin glass rods ore used for stimng If n suitable metal rack for holding the tubes Is available, all samples may bo boiled at the same time.

After coolmg the contents of the centrifuge tubes are transferred quan titatively mto Hagedom-Jenaen tubes. Whea large centrifuge tubes are used fig 24 rthe titrarion-maybe perfenned directly m the centrifuge tubes.

E-xact potassium values can only be obtained when tho scrum is completely free of hemoglobin. Principle of the mdhod Calcium is precipitated as calcium oxalate in serum or plasma.

Example ThlcMuliate used up by the blank 1 09 ml thloaolfate used up by the aample 0. In order to prevent any precipita tion of magnesium when ammomum oxalate is added, drops of acetic acid are added until the green color changes back to red.

Three ml of ammonia solu tion arc added the tubes ogam centnfuged and the supernatant removed as described above. This procedure is repeated tmee more The precipitate must not be stirred up durmg tho process of washmg Now the precipitate is dissolved m 0.

The phoephorua content of the precipitate la detenmned and from this value tho magnesium content of tlie ongmol aample a cal ciliated.

ReagenU 1 cold saturated ammonium oxalate solution 2 2 per cent ammonium phosphate Bolution 3 concentrated ammonia MflJnly according to W Donnie Journ Biol Chem 51 One mb of 10 N HiSOi ts slowlj added to the hot sample to dissolve the preerpilate After cooling it a mode up to the mati.

Now the sample is filtered through a Pregl filter fig 25a while still hot To avoid the formation of a precipitate along the walls of the filter tubing the apparatus is nnsed uith hot water immediately be- fore use.

A high magne- sium lev cl can bo encountered in mvo'Uicnla and during pcnodic paml VMS of tho extremities which maj be connected with an occasiona!

To each flask 3 ml of sodium acetate and bromme-glacml acetic acid is added from a droppmg flask until the brown color bromme persists.

After each determination the apparatus must be washed with re- distilled ivater, the water being sucked through the inner tube of the condenser with a water pump If organs are to be digested 1 ml of concentrated nitnc acid is added to the digestion acid and after the water has evaporated the sample is treated as described above.

Pfld of reagent 2 the following reagents are used reagent B HNOi concentrate p. The digestion is modifled os foUoxrs With a capillary pipet 0 Calojlation Thiosulfate value for the sample rmnus value obtained for the blank multiplied by 0 gives mg iron m the unknown sample.

Procedure One ml of HCl 1 is added to 2 ml of scrum or plasma slight hemolysis will not mtorfero m a "mnll Erlonmoy er flask or test tube, well nuxod and allowed to stand for ten mmutes.

After the addition of 2 ml of tnchlomcctio acid 2 the flask is left standing for another ten mmutes whereupon the mixture is transferred to a centnfugo tube and centnfugod The supernatant is filtered through a small iron free filter mto a graduated 10 ml gloss tube.

Between 35 and 35 ml of filtrate ore obtamed 0 05 mL of phenantrohne eolation DETEHinNATlON OF THE KATIONB 3 0 1 ml of hTdroquilione Bolution 4 and 0 05 ml of mtrophenol 6 are added to the tube xveU imxed and ammonia 6 la added alowiy drop by drop At first the pink color of the phenantrolme eomixrand appears, after a few more drops the definite deep yellow color of the mdicator can be observed At this point sulfuno acid 7 ifl added drop by drop untfl the yellow color diaappeors and the pmk color is again viaible.

The color ivill remain amstant for several hours and the reeding need not bo done immediately Two ml of standard solution 0 in a centrifuge tube are mixed with 1 mL of HCl 1 and 2 ml of tnchloracetio acid 2 , then centrifuged and filtered The some amount of this filtrate os serum filtrate obtamed M treated exactly as described above.

Values bdow C6 7 per cent and above y per cent for males and below 5o y per cent and above y pier cent for fomales must be considered pathologic Tho scrum level of iron is lowered m all ane mias with iron deficiency, often m jxTmciouB anemia during remission and m various infectious diseases It is elevated m hcanolytic processes, shortly after transfusions and after massive therapeutic iron dosage, often m iHsrnicious nnfTnin.

If very bttle scrum ts available only 2 ml of serum may be diluted with 10 ml of water and 8 ml of trichloracetic acid is added For the onaJyEiB 6 ml of flltxnto corresponding to 0.

It 10 well shaken until the red color of the solution begins to fade "Ilicn it is cooled with shaking under r unnin g water until the color turns green The supematant is decanted and the romairung mercury is washed several tunes with distilled water the nwh water bemg added each tune to the decanted supernatant The bquid is mode up to mL with dist il led water The solution should be dear, othennse it must be allowed to stand until the precipitate has settled and 13 again decanted b carbonate-free NaOH m a porcehun dish resting on a cork plate Gm of NaOH ore dissolved In Gm of water with Accordlnf to a soggoetioa of Rassol L.

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